Oral Presentation

Tissue and molecular effects of the cavitated solution in the endometrial receptivity rehabilitation of patients with the uterine infertility

Oxana Melkozerova (RU), Nadezda Bashmakova (RU), Guzel Chistyakova (RU)

[Melkozerova] Ural Research Institution of Maternity and Child Care, [Bashmakova] Ural Research Institution of Maternity and Child Care, [Chistyakova] Ural Research Institution of Maternity and Child Care

Аim. Evaluate the efficiency of granulocyte colony-stimulating factor (G-CSF) cavitated low-frequency sonication in restoring endometrial receptivity in patients with uterine infertility. Material and methods. 92 women with uterine infertility due to endometrial hypoplasia were examined in the dynamics of therapy including irrigation of the uterine cavity with cavitated low-frequency ultrasound drug solutions. The control group consisted of 28 healthy fertile women. A histological and immunohistochemical study of the endometrium was performed on the LH+7, before the start of therapy and in the next cycle after the end of treatment. Results: After treatment, 62 (67.39%) women with infertility in the endometrial specimens had adequate vascularization of the stroma (before treatment, 36.95%, p=0.017, in control 78.57%), in 68 (73.91%) women - a decrease in the density of the stromal matrix, in 64 (69.56%) - mature pinopodia were determined (initially 28.26%, p=0.0015). After therapy, the ratio of ERα/PR expression in the stroma of the implantation endometrium was normalized, which was characterized by the prevalence of the PR pool (1.01±0.34 before treatment, 0.36±0.03 after therapy, p=0.040, in control 0.34±0.06, p >0.05). The parameters of stromal expression of natural uterine killers against the background of treatment in patients with infertility come in accordance with the indices of healthy fertile women (from 35.21±2.14 to 45.75±3.18 cells l/s; in the control 47.8±2.13 cells in l/s; p >0.05). The expression level of CD34 in the endometrial stroma after the treatment was significantly increased (20.88±0.77 versus 33.83±3.63 cells in l/s, p=0.035). Conclusion. The use of G-CSF cavitaion solutions for irrigation of the uterine cavity appears to be effective in restoring endometrial receptivity in patients with uterine infertility due to endometrial hypoplasia. Key words: uterine infertility, endometrial hypoplasia, cavitation irrigation of uterine cavity, granulocyte colony-stimulating factor

 

 

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