P5. Gene expression of small leucine-rich proteoglycans (SLRPS) in lacrimal gland non-pregnant and pregnant with hyperprolactinemia induced metoclopramide

Ariadne Araujo (BR), Manuel Simoes (BR), Jose Soares-Jr (BR), Osvaldo Araujo Júnior (BR), Edmundo Baracat (BR), Regina Gomes (BR)

[Araujo] Federal University of São Paulo , [Simoes] Federal University of São Paulo, [Soares-Jr] University of São Paulo, [Araujo Júnior] Federal University of São Paulo, [Baracat] University of São Paulo, [Gomes] Federal University of São Paulo

Context: Hyperprolactinemia is a constant concern in many areas of medicine, including eye health, a multidisciplinary interest. Researchers report that prolactin can act on various body systems, in particular in the ocular system, which influences the function of lacrimal gland.Objective: This report aims to assess gene expression and immunolocalization of small leucine-rich proteoglycans, SLRPs, (class I: biglycan and decorin) and (class II: lumican and fibromodulin). Methods: 20 female mice/groups: CONTROL GROUP (Non-pregnant Ctr) received 0.2 mL of saline (vehicle) and the EXPERIMENTAL GROUP (non pregnant HPrl): 200 µg/day of metoclopramide, dissolved in vehicle. After 50 days, 10 females of each group were placed for mating with males and continued to receive treatment. The females non-pregnant were euthanasia on 50th day (EXPERIMENTAL I) and the females pregnant were euthanized on 5.5th to 6.5th post-coital day (EXPERIMENTAL II). Main Outcome Measure: The blood samples were collected for hormone measurements. The in lacrimal gland was processed for gene expression by RT-qPCR. The results were subjected to statistical test (p < 0.05). Results: Serum prolactin levels were higher in all the groups, while the levels of estradiol and progesterone were lower only in non-pregnant group compared to non-pregnant group. Gene expression showed in the gene expression of the biglycan, decorin, lumican and fibromodulin showed decrease in non-pregnant/pregnant HPrl compared to non-pregnant/pregnant Ctr, p < 0.05. Conclusion: Our data suggest that the state of hyperprolactinemia changed differently the gene expression of the small leucine-rich proteoglycans (SLRPs) in the extracellular matrix of the in lacrimal gland of pregnant and non-pregnant.