P360. Differential gene expression in the endometrium of reproductive age women with intrauterine adhesions.

Patimat Khirieva (RU), Mariya Kuznetsova (RU), Andrey Bystritskiy (RU), Sergei Martynov (RU), Dmitrii Trofimov (RU), Leila Adamyan (RU)

[Khirieva] Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation, Moscow., [Kuznetsova] Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation, Moscow., [Bystritskiy] Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation, Moscow., [Martynov] Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation, Moscow., [Trofimov] Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation, Moscow., [Adamyan] Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation, Moscow.

Objective: the search of molecular genetic markers in endometrial tissue of reproductive age women with intrauterine adhesions comparув to the endometrium of healthy women. Methods: clinical and laboratory examination, pipel-biopsy, surgical treatment, microarray transcriptome analysis (Affymetrix GeneChip Human Exon 1.0 ST). Patient(s): group 1 – patients with intrauterine adhesions (n=10), group 2 – healthy women without intrauterine adhesions (n=10). Intervention(s): surgical treatment of 20 women of reproductive age – hysteroscopic adhesiolysis in the middle stage of proliferation phase of menstrual cycle (9th day of the menstrual cycle). Main Outcome Measure(s): we found 10 genes with expression level changed 3 times and more in endometrial tissues of women with intrauterine adhesions compared to the endometrial tissues of women of group of conditionally healthy women, of which 9 genes show increased expression on case of intrauterine adhesions (S100A8, HBB, VNN2, RGS2, ERAP2, AQP9, MNDA, TUBA3E, FCGR3B) and 1 – decreased expression (NTRK3). Result(s): expression of genes S100A8, HBB, VNN2, RGS2, ERAP2, AQP9, MNDA, TUBA3E, FSGR3B is increased in endometrial tissues of women with intrauterine adhesions. Conclusion: the found differences in gene expression levels in endometrial tissues of women with intrauterine adhesions may be helpful in either study of the pathogenesis of the lesion or in designing a molecular diagnostic tool used to individualize the treatment of the lesion.