Poster Session

P262. Matrix metalloproteinase-9 and neutrophil gelatinase-associated lipocalin levels in advanced stage endometriosis

Arzu Bostancı Durmuş (TR), Gamze Sinem Çağlar (TR), Sevim Dinçer Cengiz (TR)

[Bostancı Durmuş] Ufuk Univercity Faculty of Medicine, Ankara, [Çağlar] Ufuk Univercity Faculty of Medicine, Ankara, [Dinçer Cengiz] Ufuk Univercity Faculty of Medicine, Ankara

Context:Endometriosis is a chronic disease, which is surgically diagnosed and treated.Peritoneal disease and adhesions in severe endometriosis cannot be detected without surgery.Objective:The aim of this study is to develop a non-invasive test for prediction of severe endometriosis.Methods:All cases and controls gave informed consent and accepted to give serum samples for matrix metalloproteinase-9 (MMP-9) levels and urine for neutrophil gelatinase-associated lipocalin (NGAL).Participants:Between November 2016-June 2017 surgically treated endometriosis cases at laparoscopic surgery were included in the study performed at hospital.Controls were cases undergoing laparoscopy for benign gynecological purposes without endometriosis.Intervention:Laparoscopic surgery for endometriosis was performed for infertility and/or pain.In controls infertility, tubal occlusion, hydrosalpinx, chronic pelvic pain, benign ovarian cysts were the indications for surgery.Before any intervention, blood sample for MMP-9 was collected.After anesthesia the bladder was drained and urine sample was used for uNGAL analysis.Main outcome Measures:The correlation between biochemical parameters in cases and surgical stage of the disease (American Fertility Society staging system) was documented.The predictive value of MMP/NGAL ratio for endometriosis and also the severe forms of the disease (Stage 3-4) was also analyzed.Results:The median age of the cases were 34 and the body mass index (BMI) was 26.7 kg/m2.NGAL levels did not differ significantly between groups with (n=60) and without endometriosis (n=31)(p=0,551) and between those with early stage (n=30) and advanced stage endometriosis (n=30)(p=0,232).MMP-9 levels were significantly different between the patients with and without endometriosis (p=0.002) and those with early stage and advanced stage endometriosis (p < 0.001).A similar significant difference was also observed in the comparison of the ratio of MMP-9/NGAL between the groups (p < 0.05).Among the variables (age,BMI,Ca125,NGAL,MMP-9,MMP-9/NGAL ratio), MMP-9 [OR:1.69; 95% Cl:(1.17-2.43); P=0.005] was found to be independent factor for advanced stage endometriosis.The threshold value for advanced stage endometriosis for MMP-9 is 14,13 pg/mL, with 80% sensitivity and 73,3% specificity.Conclusion:Severe endometriosis is associated with high MMP-9 levels which can help to predict advanced stage disease before surgery.